New Generalized Transducing Bacteriophage in Echerichia coli

Isolation and characterization of a new generalized transducing bacteriophage different from P1 in Escherichia coli.

A new generalized transducing bacteriophage in the Escherichia coli system was isolated and characterized. This phage, designated D108, makes clear plaques on E. coli K-10, K-12, K-12(P1kc), K-12(D6), B/r, C, and 15 T(-), and Shigella dysenteriae. The plaque of phage D108 is larger in size than that of phage P1kc. Electron-microscopic observation revealed that phages D108 and P1kc are morpholog...

Improved generalized transducing bacteriophage for Caulobacter crescentus.

Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and eliminated the requirement for irradiation of trans...

Genome sequence of a new Streptomyces coelicolor generalized transducing bacteriophage, ΦCAM.

Streptomyces coelicolor is a model system for the study of Streptomyces, a genus of bacteria responsible for the production of many clinically important antibiotics. Here we report the genome sequence of ΦCAM, a new S. coelicolor generalized transducing bacteriophage, isolated from a soil sample originating from Lincolnshire, United Kingdom. Many open reading frames within ΦCAM shared high leve...

Generalized transduction by bacteriophage P22 in Salmonella typhimurium. II. Mechanism of integration of transducing DNA.

Generalized transduction by bacteriophage P22 occurs by the transfer of DNA from one bacterium to another by means of a phage-like particle (i.e. a transducing particle) which contains bacterial DNA formed before infection (preceding paper). Transduction of a 3aP-labeled, light recipient with purified transducing particles whose DNA is labeled with 3H, zH, 16N, 13C results in the physical assoc...

Echerichia coli Biofilm Formation in Musca domestica Crops